Streptococcus agalactiae and Streptococcus pneumoniae are two medically important mucosal bacteria known to cause invasive diseases such pneumonia, meningitis, and sepsis, mostly in children and immunocompromised people. Drug resistance rise has been reported against both pathogens. There are vaccines against some serotypes of S. pneumoniae, but disease caused non-vaccine types is on the rise. Also, there are no vaccines licensed yet for prevention of S. agalactiae disease. The main objective of this study was to look for similarities withing important surface structures of both pathogens that could serve as basis for vaccine targets across the various serotypes of both pathogens.