Mosquito Microbiome Interactions

Mosquito Microbiome Interactions

Project Lead(s)
Dr.-Jewelna-E.B.-Akorli-pkvn9slqudpnlot9lqyqluofjohlwre093yn1jutmw
Senior Research Fellow
Project Background 

Disease transmission blocking is an expanding area of vector-borne disease research which seeks to understand the mechanisms that disrupt the development and limit the transmission of parasite from vector to human host. Some micro-organisms associated with mosquitoes demonstrate anti-parasitic effects independent of the mosquito immune system. Understanding this mechanism could help propose bacteria-mediated strategies for vector and disease control.

Objectives/Research Areas 

Research in the MoziBiome lab focuses on:

  • clarifying the mechanisms involved in the inter-relationship between mosquito microbiota and mosquito-borne parasites,
  • characterising mosquito-associated micro-organisms (bacteria, fungi, viruses, protists) of mosquitoes to identify the most effective combination of these for pathogen transmission-blocking,
  • understanding bionomics and acquisition of microbes by mosquito vectors for novel disease control approaches,
  • how micro-organisms influence variations in vector competence in natural mosquito populations.
Key Publications  
  1. Egyirifa RK, Akorli J. Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding. Mal J 2024, 23(1): 45-51. doi: 1186/s12936-024-04870-w
  2. Akorli EA, Ubiaru PC, Pradhan S, Akorli J and Ranford-Cartwright L. Bio-products from Serratia marcescens isolated from Ghanaian Anopheles gambiae reduce Plasmodium falciparum burden in vector mosquitoes. Front Trop Dis 2022, 3:979615. doi: 10.3389/fitd.2022.979615
  3. Amlalo GA, Akorli J, Akyea-Bobi NE, Akporh SS, Aqua-Baidoo D, Opoku M, Frempong KK, Pi-Bansa S, Boakye HA, Joannides J, Osei JHN, Pwalia R, Akorli EA, Manu A and Dadzie SK. Evidence of high frequencies of insecticide resistance mutations in Aedes aegypti (Culicidae) mosquitoes in urban Accra, Ghana: implications for insecticide-based vector control of Aedes-borne arboviral diseases. J Med Ent 2022, tjac120,doi: 10.1093/jme/tjac120
  4. Kinga H, Kengne-Ouafo JA, King SA, Egyirifa RK, Aboagye-Antwi F and Akorli J. Water physicochemical parameters and microbial composition distinguish Anopheles and Culex mosquito breeding sites: potential as ecological markers for larval source surveillance. J Med Ent 2022, tjac115, doi: 10.1093/jme/tjac115
  5. Akorli, J., Akorli, E.A., Tetteh, S.N.A. et al.Microsporidia MB is found predominantly associated with Anopheles gambiaes and Anopheles coluzzii in Ghana. Sci Rep 2021; 11, 18658. doi: 10.1038/s41598-021-98268-2
  6. Dada N, Jupatanakul N, Minard G, Short SM, Akorli J and Villegas LM. Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium. Microbiome2021; 9(1):36. doi: 1186/s40168-020-00987-7
  7. Ezemuoka LC, Akorli EA, Aboagye-Antwi F and Akorli J. Mosquito midgut Enterobacter cloacae and Serratia marcescens affect the fitness of adult female Anopheles gambiael. PLoS One 2020; 15(9): e0238931.doi:10.1371/journal.pone.0238931
  8. Akorli J, Namaali PA, Ametsi GW, Egyirifa RK and Pels NAP. Generational conservation of composition and diversity of field-acquired midgut microbiota in Anopheles gambiae sensu lato during colonization in the laboratory. Parasit & Vect 2019;12(27). doi:1186/s13071-019-3287-0
  9. Akorli J, Gendrin M, Pels NAP, Yeboah-Manu D, Christophides GK, and Wilson MD. Seasonality and locality affect the diversity of Anopheles gambiae and Anopheles coluzzii midgut microbiota from Ghana. PLoS One 2016; 11(6): e0157529. doi:1371/journal.pone.0157529

More publications are available at:

https://www.ncbi.nlm.nih.gov/myncbi/1PMDyLk0dCtAke/bibliography/public/

Team 
Seraphim N.A. Tetteh
Research Assistant
Sampson-Otoo
Research Assistant
Jeffrey Kankam
Research Assistant
Stephanie N. A.S. Addo
Research Assistant
Derrick SACKITEY
Research Assistant
Funder(s) 
Wellcome Trust Intermediate Fellowship. (220737/Z/20/Z). PI. Elucidating the mechanisms and functions of bacterial-derived mediators on Plasmodium falciparum. (2021-2026).
ANTI-VeC African Anopheles Symbiont Survey Award. (AV/AASS/009). PI. Diversity of Microsporidia MB, their co-occurrence and dynamics with bacterial communities associated with Anopheles mosquitoes. (2021-2022)