Genomics and Bioinformatics Core Facility (GBCF)

The Genomics and Bioinformatics Core Facility (GBCF) at the Noguchi Memorial Institute for Medical Research (NMIMR), University of Ghana, is dedicated to advancing genomics and bioinformatics research in Ghana and across Africa.

 

Vision:

To be a leading genomics and bioinformatics hub in Africa.

 

Mission:

To provide sequencing and bioinformatics support for disease surveillance, outbreak investigations, and diverse host and pathogen genomics studies in Ghana and the sub-region.  The facility is also committed to strengthening the public health workforce across Africa through specialized training in genomics and bioinformatics.

The GBCF is located on the ground floor of the Noguchi Advanced Research Laboratories (ARL), at NMIMR.  Our services are open to researchers, students, and other interested parties within and beyond the institute.            

 

LABORATORY STRUCTURE

The GBCF comprises two main laboratories that together provide an integrated platform supporting both wet-lab and data-driven genomics research:

 

  • Genomics laboratory (GENERAL LAB 1)

 Focuses on PCR amplification,

library preparation, and sequencing.

 

 

  • Bioinformatics laboratory

(BIOINFORMATICS)

A 20-seater capacity room dedicated to genomics data analysis, related

computational analyses and training.

 

RESOURCES

Computational Resources

The GBCF operates a high-performance computing cluster specifically designed to handle large-scale bioinformatic analyses and genomic data storage.

The 4node cluster comprises:

  • 1TB RAM,
  • 72 CPU cores per compute node
  • 600TB total storage capacity.

 

Wet Lab Resources

The Genomics Laboratory is equipped with PCR workstations, laminar flow hoods, and dedicated to master mix preparation, sample addition, and library preparation. A specific PCR workstation is exclusively used for master mix preparation. Other workstations are designated for sample addition and other library preparation procedures.

 

SERVICES & INSTRUMENTATION

The GBCF offers the following services:

Next Generation Sequencing

The Genomics Laboratory operates several illumina platforms to support diverse sequencing applications:

  • Illumina MiSeq:

 Suitable for  small-to-medium scale sequencing,  method development and pilot projects.  It provides accurate and timely data using illumina’s

Sequencing-by-Synthesis chemistry.

There are a variety of sequencing cartridges that come with the MiSeq which deliver different read lengths providing several options for the user. In addition, it offers the option of single or paired end reads to match your experimental needs.

  • Illumina NextSeq1000 and NextSeq2000

Offers  a comprehensive workflow offering compatible library prep kits, user-friendly setup, and integrated, onboard secondary analysis.

Gain access to a variety of applications including whole genome, single-cell, and whole-exome sequencing with the NextSeq1000 and NextSeq2000 sequencers

With the XLEAP Sequencing-by-Synthesis chemistry the Illumina NextSeq1000 and NextSeq2000 sequencers offer faster, higher quality, and higher throughput sequencing compared to the Illumina Miseq system. Depending on the sequencing cartridge used, the Illumina NextSeq1000/2000 has a data output range between 10-540Gb comprising about 100M to 1.8B single-end reads per run.

 

Third Generation Sequencing

The GBCF offers a variety of  Oxford Nanopore Technologies (ONT) platforms for fast, reliable, and high throughput long reads sequencing.

These platforms can be applied in large genome projects, microbiome or metagenomics studies.

The MinION Mk1B is a powerful, portable sequencing device that delivers cost-effective and real-time access to gigabases of long-read sequencing data. The device is small enough to fit in a pocket and powerful enough to deliver up to 30 Gb data per experiment. It is useful for small-to-middle scale sequencing runs/projects.

• MinION Mk1B

The MinION Mk1C combines the real-time, rapid, portable sequencing of the Mk1B with a dedicated graphics processing unit (GPU) and the additional features of a smartphone, such as a cellular modem and touch screen.

• MinION Mk1C

This nanopore sequencer is a compact benchtop system offering on-demand sequencing with integrated real-time data processing. With the capacity to run five flow cells comparable to five MinION Mk1B experiments either concurrently or individually. Its integrated, high performance data processing alleviates the need for complex and expensive IT infrastructure.

• GridION Mk1

A compact sequencer for two PromethION flow cells utilizing resources within the GridION or other suitable computational resources, such as workstations or laptops, to quickly enable accurate, high output nanopore sequencing. This allows up to two sequencing experiments to be run concurrently or individually.

• PromethION 2 Solo

 

Quality control of Nucleic Acids Analysis with Qubit Fluorometer, Agilent TapeStation, and LabChip GXII Touch HT

The Genomics Laboratory offers evaluation of nucleic acid concentration and size using the Qubit Fluorometer, Agilent TapeStation, and LabChip GXII Touch HT. These instruments provide quantitative measures of nucleic acid quality to proceed with other downstream applications.

See more on Quality control of Nucleic Acids Analysis

 

Qubit 4 fluorometer

The Invitrogen Qubit 4 fluorometer accurately and quickly measures the concentration of nucleic acids (DNA/RNA) and proteins. It can also be used to assess the integrity and quality of RNA.

 The readings when measured are interpolated onto a standard curve. This device detects fluorescent dyes in Qubit assays that are highly specific to a target molecule of interest (DNA/RNA/protein) in your sample. It can also be used to detect either double- or single-stranded DNA molecules (dsDNA or ssDNA) even in the presence of RNA or common contaminants such as salts, free nucleotides, and proteins. The dyes used only emit fluorescence when bound to their targets, even at very low concentrations (~ 0.05 ng/ul) so the readings are highly sensitive. The reaction is rapid thus enabling absorbance measurements to be carried out within a few minutes.

 

Qubit Flex fluorometer

The Qubit Flex fluorometer applies the same features and technology of the Qubit 4 fluorometer but allows for simultaneous measurements of eight samples at a time. This makes it ideal for high throughput applications.

 

Agilent TapeStation

The Agilent 4200 TapeStation system is an established automated electrophoretic tool for DNA and RNA sample quality control. The system provides true compatibility for library preparation quality control within any next-generation sequencing (NGS) workflow.

The TapeStation boasts of a wide range of assays that can analyze fragments ranging from as low as 25 bp to more than 60,000 bp.

 Assays require no more than 2ul per electrophoretic run, whether for RNA or DNA.

 Sensitivity: 20 pg/ul – 100 ng/ul

LabChip GXII Touch HT

The LabChip GXII Touch HT system with its electrophoretic separation technology provides a complete solution, enabling the rapid characterization of both protein and nucleic acid with minimal sample input. It provides an automated alternative to the traditional methods while also providing increased throughput required by most genomic workflows.

The LabChip GXII Touch can perform a wide range of analysis in as little as 42 seconds per sample, delivering data comparable to traditional electrophoresis with as much as 70X increased throughput.

Our lab utilizes the system to determine the fragment sizes of libraries and their corresponding molarities prior to loading onto Next Generation Sequencing (NGS) platforms.

Nucleic Acid Extraction

The Genomics Laboratory has an automated nucleic acid extractor. The QIAcube HT System is a robotic workstation that provides fast and reliable automated high-throughput nucleic acid extraction and purification. It is quick, cost-effective and delivers high quality nucleic acids from 24-96 samples per run.

The QIAcube HT System offers

  • Reliable DNA, RNA, and miRNA extraction from all sample types
  • Mid- to high-throughput solution with dedicated purification kits to save time and costs
  • Increased safety and reduced cross-contamination
  • Convenient, flexible and easy-to-use instrument with small footprint

Sample types include tissue, cell, blood, stool and food material.

 

PCR Amplification

The Genomics Laboratory is furnished with a wide range of thermal cyclers to run different protocols simultaneously, increase throughput, and run specialized applications.

 

Hamilton Microlab STAR Liquid Handler

The Hamilton Microlab STAR Liquid Handler impacts the scalability of our laboratory protocols/experiments; standardizing sample processing for large-scale sequencing projects, enabling high-throughput and quality data generation.

See more on the Hamilton Microlab STAR Liquid Handler

 

The Hamilton Microlab STAR is an advanced liquid-handling workstation designed for high-throughput and precision laboratory automation. Our lab utilizes this state-of-the-art system to streamline workflows, improve reproducibility, and enhance efficiency in genomics research applications.

 Key Features:

The STAR system ensures reliable liquid handling with superior pipetting accuracy, reducing variability in experiments. The platform supports a wide range of configurations, allowing for tailored solutions to meet specific research needs, from small-scale experiments to large-scale automated workflows. Equipped with both single- and multi-channel pipetting capabilities, it enables the efficient transfer of liquids in microplates, tubes, and reservoirs.

The Hamilton reduces manual errors and enhances throughput, making it ideal for applications such as NGS library preparation and quality control set-ups.

It is equipped with additional laboratory automation tools, including barcode scanners, on-deck thermocyclers, robotic arms, and storage modules for seamless workflow integration.

 Applications in Our Lab

The Hamilton Microlab STAR is utilized exclusively for library preparation procedures within the Genomics lab. The currently installed library preparation protocols on the STAR liquid handler include:

  • Illumina:
    • DNA Prep
    • CovidSeq
    • RNA Prep with Enrichment
  • Oxford Nanopore Technologies:
    • Midnight RT-PCR Expansion
    • Ligation Sequencing
  • Paragon:
    • Cleenplex Malaria (MadHatter)

Additionally, the following supporting methods are integrated to enhance automation:

  • Qubit Setup: Preparation of Qubit solutions for nucleic acid quantification.
  • Normalization Method: Adjustment of initial nucleic acid extract or amplicon product concentrations to target levels.
  • 96-Multipipette Head Transfer: High-throughput liquid transfer for efficient sample processing.
Microplate microcentrifuge
MixMate
Heat Block
QuantStudio 5
Centrifuges
Centrifuges

 

Guidelines on access and use of the GBCF

We provide services to researchers, clinicians, students, and other interested individuals within and beyond the Noguchi Memorial Institute for Medical Research. The GBCF is managed by a team of research assistants under the leadership of a senior member, who reports to the Director of NMIMR.

To ensure the smooth operation of the facility and maintain high research standards, all users are kindly required to adhere to the GBCF guidelines:

  • Complete the sequencing request form
  • Upon completion, sequencing request forms are reviewed; all queries are resolved between the requester and GBCF
  • A proforma invoice is issued by GBCF, endorsed by NMIMR accounts office and forwarded to the requester
  • The requester makes payment for the service and submits proof of payment to the GBCF.
  • The requester downloads and completes the sample submission form
  • The sample submission form should be submitted together with the samples according to the appropriate criteria indicated
  • Samples are received at the general laboratory according to NMIMR’s standard operating procedures
  • Samples will be checked using the sample acceptance/rejection criteria
  • Samples which fail to meet the indicated criteria will be rejected and communicated to the requester via email.

Note:

  • Click here to access the sequencing request form to request our facility’s services.
  • Download the sequencing request form template and complete it.
  • Click here to access the sample submission form.
  • Download the sample submission form and complete it.
  • Kindly forward the completed forms to the GBCF via email (genomicscorefacility@noguchi.ug.edu.gh).

Access and Training

Access to the GBCF is electronically controlled and is accessible to staff who have been trained and authorized to work in the genomics and bioinformatics laboratories.

The GBCF hosts and assists students who are carrying out their project work that is related to genomics and bioinformatics. Such individuals are trained by the GBCF staff on the relevant instruments before use.

 Terms and Conditions

To access our services, kindly refer to “Guidelines for access and use of the GBCF”

NB: Apart from statutory public holidays and weekends, the GBCF is open between the hours of 8:00 am and 5:00 pm daily.

Kindly note that the GBCF reserves the right to the following:

  • Discard any sample as per our sample rejection criteria outlined unless the sender requests their return in writing. Any associated cost regarding the return of rejected samples shall be solely borne by the sender and not the GBCF.
  • Reject requests where request forms are incorrectly or illegibly completed (if handwritten).

 

Time Limits for Additional Examination Requests

Additional requests must be received within a week of original receipt of specimens in the laboratory. Where applicable, the requester must call the laboratory for the specific test and make provision for the extra cost.

 Use of Outsourced Laboratories

The GBCF is in the process of establishing agreements with referral laboratories for outsourcing examinations it cannot perform upon receipt of samples. However, we currently do not outsource any of our examinations to any external facility.

Laboratory Reports

All client reports/results are electronically generated and sent via email.

Confidentiality of Service User Information

Clients’ information is maintained as confidential according to the information security policy of the NMIMR. Client reports/results are released ONLY to the Email address provided by the client and hard copies retrieved on request following proper identification.

 

Training and Capacity Building

The GBCF has been a major center for genomics and bioinformatics capacity building within the Africa sub-region. We have trained many laboratory personnel from over 40 different countries including Ghana, Cape Verde, Togo, Benin, Guinea, Sierra Leone, Ivory Coast, Mauritania, Liberia, etc., in genomic sequencing and bioinformatics data analysis. Our trainings are organized either independently or in collaboration with institutions such as the Africa CDC/Africa-PGI, World Health Organisation, African Society for Laboratory Medicine, the UK Health Security Agency New Variant Assessment Platform (UKHSA NVAP), and the University of Cambridge Bioinformatics Training Facility (BTF), etc.

 

Excerpts of trainings held at the Genomics and Bioinformatics Core Facility

About 140 trainees from over 40 different countries across Africa have been trained since 2021

 

 

CONTACT INFORMATION

Head, GBCF Bright Adu, Ph.D

Senior Research Fellow

Email: badu@noguchi.ug.edu.gh

Office: Noguchi Advanced Research Laboratories, Ground Floor, Room 4, Noguchi Memorial Institute for Medical Research (NMIMR), University of Ghana, Legon.

 

 

CONTACT ADDRESS

Genomics and Bioinformatics Core Facility

Noguchi Advanced Research Laboratories

Noguchi Memorial Institute for Medical Research

Akilagpa Sawyerr Road, Off South Legon Drive

University of Ghana, P. O. Box LG 581

Legon, Ghana, Legon

For enquiries please contact us through the institute on info@noguchi.ug.edu.gh or at genomicscorefacility@noguchi.ug.edu.gh

 

Acknowledgements